中国水稻科学

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (5): 633-636.DOI: 10.3969/j.issn.10017216.2012.05.019

• 研究简报 • 上一篇    

体外试验评价转基因大米Bt63的安全性

陈吴健1,郑伟 2,* ,吕沁风 2 ,张明哲1 ,吴忠华 2 ,徐琦 2 ,吴志毅 1,李禾2   

  1. 1 浙江省检验检疫科学技术研究院， 浙江 杭州 310016； 2 浙江国际旅行卫生保健中心， 浙江 杭州 310016；
  • 收稿日期:2011-10-26 修回日期:2012-12-09 出版日期:2012-09-10 发布日期:2012-09-10
  • 通讯作者: 郑伟2,*,
  • 基金资助:

    国家质检总局科技计划资助项目（2009IK162）。

Safety Assessment of Bt63, a Genetically Modified Rice in vitro

CHEN Wujian 1,  ZHENG Wei 2,* , LU Qinfeng 2 , ZHANG Mingzhe 1 , WU  Zhonghua 2 , XU Qi 2 , WU Zhiyi 1 , LI  He 2   

  1. 1 Zhejiang Academy of Science & Technology for Inspection & Quarantine, Hangzhou 310016, China; 2 Zhejiang International Travel Healthcare Center, Hangzhou 310016, China;
  • Received:2011-10-26 Revised:2012-12-09 Online:2012-09-10 Published:2012-09-10
  • Contact: ZHENG Wei2,*,

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摘要： 用不同体外实验评价了转基因大米Bt63的细胞毒性和遗传毒性。分别以200、100、50、25、12.5 μg/mL转基因大米Bt63全蛋白作用于人淋巴细胞，并各培养1.5、6、24 h。然后用彗星试验检测DNA损伤，用微核试验检测染色体畸变，用CCK8及中性红试验检测细胞毒性损伤。在经过不同的培养时间后，阳性对照组淋巴细胞的遗传及细胞损伤指标与对照组相比存在显著差异。其中，CCK8试验、中性红试验测得细胞存活率存在着明显的损伤作用时间效应关系。转基因大米Bt63全蛋白暴露组淋巴细胞遗传及细胞损伤指标与非转基因大米全蛋白暴露组相比无明显差异，且与对照差异不显著。转基因大米Bt63与非转基因大米两者在遗传毒性及细胞毒性上性状相似，实质等同。转基因大米Bt63对人淋巴细胞无明显遗传毒性及细胞毒性作用。另外，该实验体系与传统的毒理学方法相比，经济、简便、快速，具有较高的实用价值。

关键词: 转基因水稻, 体外试验, 安全性评价

Abstract: To investigate the cytogenotoxicity of Bt63, a genetically modified(GM) rice in human lymphocytes  in vitro,  human lymphocytes were exposed to whole protein of Bt63 at doses of 200, 100, 50, 25, 12.5 μg/mL and incubated for 1.5 h, 6 h and 24 h, respectively. The DNA damage, chromosomal aberrations and cytotoxicity induced by whole protein of Bt63 were measured by comet assay, micronucleus test, CCK8 assay and neutral red uptake (NRU) assay. After different incubation periods, the cytogenotoxicity of lymphocytes in positive control group was significant higher than that of lymphocytes in blank control group. Moreover, the exposure timeeffect relationship was observed in positive control group by CCK8 assay and neutral red uptake (NRU) assay. There was no significant difference in cytogenotoxicity between Bt63 group and nonGM rice group. Also, the Bt63 GM rice group did not show higher cytogenotoxicity than that of nonGM rice group, indicating that Bt63 GM rice has similar cytogenotoxicity with nonGM rice, and Bt63 GM rice has no cytogenotoxicity effect on human lymphocytes in vitro.  Compared with traditional toxicological  methods, the experimental system is economic, simple, rapid and reliable. 

Key words: genetically modified rice, in vitro test, safety assessment

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